Two-photon fluorescence isotropic-single-objective microscopy
نویسندگان
چکیده
منابع مشابه
Two-photon fluorescence isotropic-single-objective microscopy.
Two-photon excitation provides efficient optical sectioning in three-dimensional fluorescence microscopy, independently of a confocal detection. In two-photon laser-scanning microscopy, the image resolution is governed by the volume of the excitation light spot, which is obtained by focusing the incident laser beam through the objective lens of the microscope. The light spot being strongly elon...
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ژورنال
عنوان ژورنال: Optics Letters
سال: 2011
ISSN: 0146-9592,1539-4794
DOI: 10.1364/ol.37.000085